| Eaglesfield Community Church | Burlington |
| East Plains United Church | Burlington |
| Ebenezer Canadian Reformed Church | Burlington |
| Ebenezer United Church | Milton |
| Edgewater Senior Living | Georgetown |
| Edward's Pharmacy, Pharmacy Services | Oakville |
| Elder Abuse Prevention Ontario | Etobicoke Central |
| ElderDog Canada, Halton Pawd - No Physical Address | Halton Region |
| Elite Home Nursing Care, In-Home Care Services | Georgetown |
| Elizz by SE Health, Elizz Help at Home Services | Hamilton |
| Emfy Health, Home Health Care Services | Mississauga |
| End of Life Doula Association of Canada, End of Life Doula Association of Canada Resources and Doula Network | |
| Enhanced Mobility | Oakville |
| Epilepsy South Central Ontario, Mississauga | Mississauga |
| Equilibrium, Oakville, Mood Disorder Support Group | Oakville |
| Eramosa Nonprofit Housing Corporation | Guelph-Eramosa |
| Ernestine's Women's Shelter | Toronto |
| Escudero Medical Centre | Oakville |
| Esquesing Historical Society | Georgetown |
| Estate Administration and Wills, Halton Region | Halton Region |
| Evangel Family Church | Acton |
| Evangel Pentecostal Church | Oakville |
| Extendicare Burloak, Long Term Care Home | Burlington |
| Extendicare Halton Hills, Long-Term Care Centre | Georgetown |
| Extendicare Northridge, Long Term Care Centre | Oakville |
| Extendicare West Oak Village, Long Term Care Centre | Oakville |
